Dupont Lateral Flow System Listeria Test Kit

How the DuPont™ Lateral Flow System™ works The DuPont™ Lateral Flow System™ test is an immunoassay with built-in controls. It employs a unique combination of anti-Listeria antibodies and a highly engineered colloidal gold conjugate coated on the surface of a membrane. The sample wicks up the test strip by capillary action. If Listeria is present in the sample, two red lines are formed. The DuPont™ Lateral Flow System™ Listeria media provides a single-stage enrichment, with no transfers required. Simply mix the powdered media base with sterile water and add the supplement before use for results in 40 hours. After enrichment, heat an aliquot of sample broth in a test tube for 5 minutes, and allow to cool. Drop the test strip into the sample tube, and read the results 10 minutes later. One control line is built into the lateral flow strip so you know the test has worked correctly. Two lines indicate a positive result. Test strips and media base can be stored at room temperature. The media supplement requires refrigeration. Applications The DuPont™ Lateral Flow System™ Listeria test is designed to detect Listeria on environmental surfaces and in a variety of ready-to-eat foods (such as deli meats, chicken nuggets, pepperoni, hot dogs, chicken salad and potato salad), dairy product (such as ice cream, soft cheese and milk), and fish products (such as cooked shrimp and smoked fish). The test can reliabably detect 1 cfu in 25 grams of sample. Full Product Line DuPont™ Lateral Flow System™ Listeria test strips are available in 45-test kits, which include a package insert. Media base with supplement are available separately. Approvals The DuPont™ Lateral Flow System™ Listeria test has been submitted to AOAC for certification as a Performance Testedsm Method.